GPRC6A Polyclonal Antibody
- 货号：YT2040
- 应用：WB;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>NOD-like receptor signaling pathway
- 蛋白名称：
- G-protein coupled receptor family C group 6 member A
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GPRC6A. AA range:471-520
- 特异性：
- GPRC6A Polyclonal Antibody detects endogenous levels of GPRC6A protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GPRC6A;G-protein coupled receptor family C group 6 member A;hGPRC6A;G-protein coupled receptor GPCR33;hGPCR33
- 背景：
- Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain (summary by Pi et al., 2005 [PubMed 16199532]).[supplied by OMIM, Nov 2010],
- 功能：
- function:Receptor that is activated by both amino acids and extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. Senses changes in the extracellular concentration of calcium ions, suggesting that it may mediate extracellular calcium-sensing responses in osteoblasts. Osteocalin, stimulates its activity in presence of calcium. Has a lower affinity for calcium than CASR. Also acts as a receptor for amino acids, with a preference for basic amino acids such as L-Lys, L-Arg and L-ornithine. Its affinity for amino acids suggests that it may act as a regulatory component of the urea cycle.,PTM:N-glycosylated.,similarity:Belongs to the G-protein coupled receptor 3 family.,subunit:Homodimer; disulfide-linked.,tissue specificity:Widely expressed. Expressed at high level in brain,
- 细胞定位：
- Cell membrane ; Multi-pass membrane protein .
- 组织表达：
- Isoform 1 is expressed at high level in brain, skeletal muscle, testis, bone, calvaria, osteoblasts and leukocytes. Expressed at intermediate level in liver, heart, kidney and spleen. Expressed at low level in lung, pancreas, placenta and ovary. Not detected in thymus, prostate, small intestine, tongue and colon. Isoform 1 and isoform 2 are expressed in kidney at the same level. Isoform 2 is expressed at lower level than isoform 1 in the other tissues.
- Western Blot analysis of Jurkat cells using GPRC6A Polyclonal Antibody diluted at 1:1000
- Western Blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
- Immunofluorescence analysis of MCF7 cells, using GPRC6A Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using GPRC6A Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using GPRC6A antibody.
