GPR18 Polyclonal Antibody
- 货号:YT2005
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- N-arachidonyl glycine receptor
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human GPR18. AA range:191-240
- 特异性:
- GPR18 Polyclonal Antibody detects endogenous levels of GPR18 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- GPR18;GPCRW;N-arachidonyl glycine receptor;NAGly receptor;G-protein coupled receptor 18
- 背景:
- function:Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells.,
- 功能:
- function:Receptor for N-arachidonyl glycine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. May contribute to regulation of the immune system.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Most abundant in testis and spleen. Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells.,
- 细胞定位:
- Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane .
- 组织表达:
- Expressed in midpiece of spermatozoon (at protein level) (PubMed:27572937). Most abundant in testis and spleen (PubMed:16844083). Highly expressed in CD4 and CD8-positive T-cells as well as CD19-positive B-cells (PubMed:16844083).
![](/uploadfiles/PIC/yt2005_pic848318522.jpg)
- Immunofluorescence analysis of HUVEC cells, using GPR18 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2005_pic848418522.jpg)
- Western blot analysis of lysates from 293 and Jurkat cells, using GPR18 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt2005_pic994318522.jpg)
- Western blot analysis of the lysates from HUVECcells using GPR18 antibody.
![](/uploadfiles/PIC/7-IHC-biantaotimm202207.jpg)
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).