Golgin 45 Polyclonal Antibody
- 货号：YT1942
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human BLZF1. AA range:10-59
- 特异性：
- Golgin 45 Polyclonal Antibody detects endogenous levels of Golgin 45 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- BLZF1;JEM1;Golgin-45;Basic leucine zipper nuclear factor 1;JEM-1;p45 basic leucine-zipper nuclear factor
- 背景：
- caution:Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.,function:Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.,induction:Up-regulated by retinoids.,subunit:Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi.,tissue specificity:Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.,
- 功能：
- caution:Because of the presence of a potential basic motif and leucine-zipper domain, PubMed:9129147 and PubMed:11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing.,function:Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.,induction:Up-regulated by retinoids.,subunit:Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi.,tissue specificity:Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines,
- 细胞定位：
- Golgi apparatus membrane .; [Isoform 1]: Nucleus . Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm. .; [Isoform 2]: Cytoplasm . Not detected in the nucleus. .
- 组织表达：
- Detected in adrenal gland (PubMed:9129147).
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
- Western blot analysis of lysates from Jurkat cells, using BLZF1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from COLO205 cells using BLZF1 antibody.
