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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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GNL1 Polyclonal Antibody
- 货号:YT1937
- 应用:WB;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Guanine nucleotide-binding protein-like 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human GNL1. AA range:61-110
- 特异性:
- GNL1 Polyclonal Antibody detects endogenous levels of GNL1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- GNL1;HSR1;Guanine nucleotide-binding protein-like 1;GTP-binding protein HSR1
- 背景:
- The GNL1 gene, identified in the human major histocompatibility complex class I region, shows a high degree of similarity with its mouse counterpart. The GNL1 gene is located less than 2 kb centromeric to HLA-E, in the same transcriptional orientation. GNL1 is telomeric to HLA-B and HLA-C. [provided by RefSeq, Jul 2008],
- 功能:
- domain:In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.,function:Possible regulatory or functional link with the histocompatibility cluster.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MMR1/HSR1 GTP-binding protein family.,
- 细胞定位:
- extracellular space,nucleus,
- 组织表达:
- Cervix,Muscle,Peripheral blood leukocyte,Skin,T-cell,
![](/uploadfiles/PIC/yt1937-wb.jpg)
- Western Blot analysis of various cells using GNL1 Polyclonal Antibody
![](/uploadfiles/PIC/yt1937-wb(2).jpg)
- Western Blot analysis of K562 cells using GNL1 Polyclonal Antibody
![](/uploadfiles/PIC/yt1937_pic703718522.jpg)
- Western blot analysis of lysates from Jurkat, LOVO, and K562 cells, using GNL1 Antibody. The lane on the right is blocked with the synthesized peptide.