GK1/3 Polyclonal Antibody
- 货号：YT1911
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Glycerolipid metabolism;>>Metabolic pathways;>>PPAR signaling pathway
- 蛋白名称：
- Putative glycerol kinase 3
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GK3. AA range:21-70
- 特异性：
- GK1/3 Polyclonal Antibody detects endogenous levels of GK1/3 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GK;Glycerol kinase;GK;Glycerokinase;ATP:glycerol 3-phosphotransferase;GK3P;GKP3;GKTB;Putative glycerol kinase 3;GK 3;Glycerokinase 3;ATP:glycerol 3-phosphotransferase 3;Glycerol kinase; testis specific 1
- 背景：
- The protein encoded by this gene belongs to the FGGY kinase family. This protein is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Mutations in this gene are associated with glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011],
- 功能：
- catalytic activity:ATP + glycerol = ADP + sn-glycerol 3-phosphate.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,disease:Defects in GK are the cause of GK deficiency (GKD) [MIM:307030]. This disease can be either symptomatic with episodic metabolic and CNS decompensation or asymptomatic with hyperglycerolemia and hyperglyceroluria only.,function:Key enzyme in the regulation of glycerol uptake and metabolism.,pathway:Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.,similarity:Belongs to the FGGY kinase family.,subcellular location:In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.,tissue specificity:Highly expressed in the liver, kidney and testis
- 细胞定位：
- Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm.
- 组织表达：
- Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver.
- Western Blot analysis of various cells using GK1/3 Polyclonal Antibody
- Western blot analysis of lysates from 293 cells, using GK3 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
