GIMAP4 Polyclonal Antibody
- 货号:YT1904
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- GTPase IMAP family member 4
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human GIMAP4. AA range:131-180
- 特异性:
- GIMAP4 Polyclonal Antibody detects endogenous levels of GIMAP4 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- GIMAP4;IAN1;IMAP4;MSTP062;GTPase IMAP family member 4;Immunity-associated nucleotide 1 protein;IAN-1;hIAN1;Immunity-associated protein 4
- 背景:
- This gene encodes a protein belonging to the GTP-binding superfamily and to the immuno-associated nucleotide (IAN) subfamily of nucleotide-binding proteins. The encoded protein of this gene may be negatively regulated by T-cell acute lymphocytic leukemia 1 (TAL1). In humans, the IAN subfamily genes are located in a cluster at 7q36.1. [provided by RefSeq, Jul 2008],
- 功能:
- function:Exhibits intrisinic GTPase activity. Shows a higher affinity for GDP over GTP (about 12-fold higher), and binding shows an absolute requirement for magnesium.,similarity:Belongs to the IAN GTP-binding protein family.,tissue specificity:Highly expressed in spleen and peripheral blood leukocytes that contain mostly T- and B-lymphocytes. Expressed specifically in resting T- and B-lymphocytes and expression significantly decreases during B- or T-lymphocyte activation. Expressed at lower levels in thymus, ovary, colon and small intestine.,
- 细胞定位:
- Cytoplasm, cytosol .
- 组织表达:
- Highly expressed in spleen and peripheral blood leukocytes that contain mostly T- and B-lymphocytes. Expressed specifically in resting T- and B-lymphocytes and expression significantly decreases during B- or T-lymphocyte activation. Expressed at lower levels in thymus, ovary, colon and small intestine.
![](/uploadfiles/PIC/yt1904-wb.jpg)
- Western Blot analysis of various cells using GIMAP4 Polyclonal Antibody
![](/uploadfiles/PIC/yt1904_pic702018522.jpg)
- Western blot analysis of lysates from HeLa, HT-29, and RAW264.7 cells, using GIMAP4 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1904_pic990918522.jpg)
- Western blot analysis of the lysates from COLO205 cells using GIMAP4 antibody.
![](/uploadfiles/PIC/43-(2)biantaoticc.jpg)
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).