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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
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GCP6 Polyclonal Antibody
- 货号:YT1882
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Gamma-tubulin complex component 6
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TUBGCP6. AA range:741-790
- 特异性:
- GCP6 Polyclonal Antibody detects endogenous levels of GCP6 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TUBGCP6;GCP6;KIAA1669;Gamma-tubulin complex component 6;GCP-6
- 背景:
- The protein encoded by this gene is part of a large multisubunit complex required for microtubule nucleation at the centrosome. [provided by RefSeq, Jul 2008],
- 功能:
- function:Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.,sequence caution:Intron retention.,similarity:Belongs to the GCP family.,subunit:Gamma-tubulin complex is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6.,
- 细胞定位:
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .
- 组织表达:
- Brain,Epithelium,Kidney,PCR rescued clones,
![](/uploadfiles/PIC/yt1882-wb.jpg)
- Western Blot analysis of various cells using GCP6 Polyclonal Antibody diluted at 1:1000
![](/uploadfiles/PIC/yt1882-ihc-201.jpg)
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
![](/uploadfiles/PIC/yt1882_pic698118522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TUBGCP6 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1882_pic698218522.jpg)
- Western blot analysis of lysates from COLO cells, using TUBGCP6 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1882_pic1075018522.jpg)
- Western blot analysis of the lysates from HeLa cells using TUBGCP6 antibody.