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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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GCP5 Polyclonal Antibody
- 货号:YT1881
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Gamma-tubulin complex component 5
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TUBGCP5. AA range:741-790
- 特异性:
- GCP5 Polyclonal Antibody detects endogenous levels of GCP5 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TUBGCP5;GCP5;KIAA1899;Gamma-tubulin complex component 5;GCP-5
- 背景:
- function:Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.,similarity:Belongs to the GCP family.,subunit:Gamma-tubulin complex is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6.,tissue specificity:Widely expressed, with highest levels in heart and skeletal muscle and moderate levels in brain.,
- 功能:
- function:Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.,similarity:Belongs to the GCP family.,subunit:Gamma-tubulin complex is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6.,tissue specificity:Widely expressed, with highest levels in heart and skeletal muscle and moderate levels in brain.,
- 细胞定位:
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .
- 组织表达:
- Widely expressed, with highest levels in heart and skeletal muscle and moderate levels in brain.
![](/uploadfiles/PIC/yt1881-wb.jpg)
- Western Blot analysis of various cells using GCP5 Polyclonal Antibody diluted at 1:2000
![](/uploadfiles/PIC/yt1881-wb(2).jpg)
- Western Blot analysis of 293 cells using GCP5 Polyclonal Antibody diluted at 1:2000
![](/uploadfiles/PIC/yt1881wb42174.jpg)
- Western blot analysis of Mouse-kidney mouse-brain HELA KB SH-SY5Y 293T lysis using GCP5 antibody. Antibody was diluted at 1:2000
![](/uploadfiles/PIC/yt1881-ihc-209.jpg)
- Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
![](/uploadfiles/PIC/yt1881_pic698018522.jpg)
- Western blot analysis of lysates from HT-29, Jurkat, and 293 cells, using TUBGCP5 Antibody. The lane on the right is blocked with the synthesized peptide.