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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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GCP3 Polyclonal Antibody
- 货号:YT1879
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Gamma-tubulin complex component 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TUBGCP3. AA range:201-250
- 特异性:
- GCP3 Polyclonal Antibody detects endogenous levels of GCP3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TUBGCP3;GCP3;Gamma-tubulin complex component 3;GCP-3;hGCP3;Gamma-ring complex protein 104 kDa;h104p;hGrip104;Spindle pole body protein Spc98 homolog;hSpc98
- 背景:
- alternative products:Experimental confirmation may be lacking for some isoforms,function:Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.,similarity:Belongs to the GCP family.,subunit:Gamma-tubulin complex is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6.,tissue specificity:Ubiquitously expressed.,
- 功能:
- alternative products:Experimental confirmation may be lacking for some isoforms,function:Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.,similarity:Belongs to the GCP family.,subunit:Gamma-tubulin complex is composed of gamma-tubulin, GCP2, GCP3, GCP4, GCP5 and GCP6.,tissue specificity:Ubiquitously expressed.,
- 细胞定位:
- Cytoplasm, cytoskeleton, microtubule organizing center, centrosome .
- 组织表达:
- Ubiquitously expressed.
![](/uploadfiles/PIC/yt1879-wb.jpg)
- Western Blot analysis of various cells using GCP3 Polyclonal Antibody
![](/uploadfiles/PIC/yt1879-wb(2).jpg)
- Western Blot analysis of K562 cells using GCP3 Polyclonal Antibody
![](/uploadfiles/PIC/yt1879_pic697818522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TUBGCP3 Antibody. The picture on the right is blocked with the synthesized peptide.