GBDR1 Polyclonal Antibody
- 货号：YT1866
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Ubiquitin-associated domain-containing protein 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human UBAC1. AA range:91-140
- 特异性：
- GBDR1 Polyclonal Antibody detects endogenous levels of GBDR1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- UBAC1;GBDR1;KPC2;UBADC1;Ubiquitin-associated domain-containing protein 1;UBA domain-containing protein 1;E3 ubiquitin-protein ligase subunit KPC2;Glialblastoma cell differentiation-related protein 1;Kip1 ubiquitination-promoting com
- 背景：
- function:Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 STI1 domain.,similarity:Contains 1 ubiquitin-like domain.,similarity:Contains 2 UBA domains.,subunit:Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2. Interacts with RNF123 via its N-terminal domain. Interacts with the proteasome via its N-terminal domain.,tissue specificity:Ubiquitous.,
- 功能：
- function:Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 STI1 domain.,similarity:Contains 1 ubiquitin-like domain.,similarity:Contains 2 UBA domains.,subunit:Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2. Interacts with RNF123 via its N-terminal domain. Interacts with the proteasome via its N-terminal domain.,tissue specificity:Ubiquitous.,
- Western Blot analysis of various cells using GBDR1 Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from 293 and HUVEC cells, using UBAC1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HT-29 cells using UBAC1 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
