GATA-1 Polyclonal Antibody
- 货号：YT1858
- 应用：WB;IHC;IP;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Erythroid transcription factor
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GATA1. AA range:109-158
- 特异性：
- GATA-1 Polyclonal Antibody detects endogenous levels of GATA-1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GATA1;ERYF1;GF1;Erythroid transcription factor;Eryf1;GATA-binding factor 1;GATA-1;GF-1;NF-E1 DNA-binding protein
- 背景：
- This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008],
- 功能：
- disease:Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.,disease:Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also called thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. The disease consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced (hemo)globin chain synthesis resembling that of beta-thalassemia minor.,domain:The two fingers are functionally distinct and cooperate to achie
- Western Blot analysis of various cells using GATA-1 Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using GATA1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from COLO cells, using GATA1 Antibody. The lane on the right is blocked with the synthesized peptide.
