GALK1 Polyclonal Antibody
- 货号：YT1842
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Galactose metabolism;>>Amino sugar and nucleotide sugar metabolism;>>Metabolic pathways;>>Biosynthesis of nucleotide sugars
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GALK1. AA range:1-50
- 特异性：
- GALK1 Polyclonal Antibody detects endogenous levels of GALK1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GALK1;GALK;Galactokinase;Galactose kinase
- 背景：
- Galactokinase is a major enzyme for the metabolism of galactose and its deficiency causes congenital cataracts during infancy and presenile cataracts in the adult population. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate.,disease:Defects in GALK1 are the cause of galactosemia II [MIM:230200]. It is an autosomal recessive deficiency characterized by congenital cataracts during infancy and presenile cataracts in the adult population. The cataracts are secondary to accumulation of galactitol in the lenses.,function:Major enzyme for galactose metabolism.,pathway:Carbohydrate metabolism; galactose metabolism.,similarity:Belongs to the GHMP kinase family.,similarity:Belongs to the GHMP kinase family. GalK subfamily.,
- 细胞定位：
- cytoplasm,cytosol,membrane,extracellular exosome,
- 组织表达：
- Blood,Brain,Fetal brain cortex,Lung,
- Western Blot analysis of various cells using GALK1 Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from HUVEC and HeLa cells, using GALK1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
