Galectin-8 Polyclonal Antibody
- 货号：YT1840
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human LEG8. AA range:61-110
- 特异性：
- Galectin-8 Polyclonal Antibody detects endogenous levels of Galectin-8 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000. IF 1:100-300 Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- LGALS8;Galectin-8;Gal-8;Po66 carbohydrate-binding protein;Po66-CBP;Prostate carcinoma tumor antigen 1;PCTA-1
- 背景：
- This gene encodes a member of the galectin family. Galectins are beta-galactoside-binding animal lectins with conserved carbohydrate recognition domains. The galectins have been implicated in many essential functions including development, differentiation, cell-cell adhesion, cell-matrix interaction, growth regulation, apoptosis, and RNA splicing. This gene is widely expressed in tumoral tissues and seems to be involved in integrin-like cell interactions. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],
- 功能：
- domain:Contains two homologous but distinct carbohydrate-binding domains.,online information:Galectin-8 C-terminal CRD,online information:Galectin-8 long isoform,similarity:Contains 2 galectin domains.,tissue specificity:Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.,
- 细胞定位：
- Cytoplasmic vesicle . Cytoplasm, cytosol .
- 组织表达：
- Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
- Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
- Western Blot analysis of 3T3 cells using Galectin-8 Polyclonal Antibody diluted at 1:1000
- Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma tissue, using LEG8 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from NIH/3T3 cells, using LEG8 Antibody. The lane on the right is blocked with the synthesized peptide.
