Frizzled-3 Polyclonal Antibody
- 货号:YT1777
- 应用:WB;IF;ELISA
- 种属:Human;Mouse
- 简介:
- >>mTOR signaling pathway;>>Wnt signaling pathway;>>Axon guidance;>>Hippo signaling pathway;>>Signaling pathways regulating pluripotency of stem cells;>>Melanogenesis;>>Cushing syndrome;>>Alzheimer disease;>>Pathways of neurodegeneration - multiple diseases;>>Human papillomavirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Basal cell carcinoma;>>Breast cancer;>>Hepatocellular carcinoma;>>Gastric cancer
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human FZD3. AA range:141-190
- 特异性:
- Frizzled-3 Polyclonal Antibody detects endogenous levels of Frizzled-3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- FZD3;Frizzled-3;Fz-3;hFz3
- 背景:
- frizzled class receptor 3(FZD3) Homo sapiens This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010],
- 功能:
- domain:Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway.,domain:The FZ domain is involved in binding with Wnt ligands.,function:Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphog
- 细胞定位:
- Membrane; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein . Cell surface . Apical cell membrane ; Multi-pass membrane protein. Colocalizes with FZD6 at the apical face of the cell (By similarity). .
- 组织表达:
- Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.
![](/uploadfiles/PIC/yt1777-wb.jpg)
- Western Blot analysis of various cells using Frizzled-3 Polyclonal Antibody
![](/uploadfiles/PIC/yt1777-wb(2).jpg)
- Western Blot analysis of RAW264.7 cells using Frizzled-3 Polyclonal Antibody
![](/uploadfiles/PIC/yt1777_pic828618522.jpg)
- Immunofluorescence analysis of COS7 cells, using FZD3 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1777_pic828718522.jpg)
- Western blot analysis of lysates from A549 and RAW264.7 cells, using FZD3 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1777_pic988518522.jpg)
- Western blot analysis of the lysates from HeLa cells using FZD3 antibody.