FoxO4 Polyclonal Antibody
- 货号：YT1764
- 应用：WB;IF;ELISA
- 种属：Human;Mouse;Monkey
- 简介：
- >>Ras signaling pathway;>>FoxO signaling pathway;>>Shigellosis
- 蛋白名称：
- Forkhead box protein O4
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human FOXO4. AA range:417-466
- 特异性：
- FoxO4 Polyclonal Antibody detects endogenous levels of FoxO4 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- FOXO4;AFX;AFX1;MLLT7;Forkhead box protein O4;Fork head domain transcription factor AFX1
- 背景：
- This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010],
- 功能：
- disease:A chromosomal aberration involving FOXO4 is found in acute leukemias. Translocation t(X;11)(q13;q23) with MLL/HRX. The result is a rogue activator protein.,function:Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.,pharmaceutical:A constitutively active FOXO4 mutant where phosphorylation sites Thr-32, Ser-187 and Ser-262 have been mutated to alanine may have therapeutic potential in ERBB2/HER2-overexpressing cancers as it inhibits ERBB2-mediated cell survival, transformation and tumorigenicity.,PTM:Acetylation by CBP, which is induced by peroxidase stress, inhibits transcriptional activity. Dea
- 细胞定位：
- Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.
- 组织表达：
- Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.
- Western Blot analysis of various cells using FoxO4 Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using FOXO4 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from K562 cells, using FOXO4 Antibody. The lane on the right is blocked with the synthesized peptide.
