Fos B Polyclonal Antibody
- 货号：YT1738
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Monkey
- 简介：
- >>Osteoclast differentiation;>>IL-17 signaling pathway;>>Cocaine addiction;>>Amphetamine addiction;>>Alcoholism
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human FosB. AA range:12-61
- 特异性：
- Fos B Polyclonal Antibody detects endogenous levels of Fos B protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- FOSB;G0S3;Protein fosB;G0/G1 switch regulatory protein 3
- 背景：
- The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- 功能：
- function:FosB interacts with Jun proteins enhancing their DNA binding activity.,similarity:Belongs to the bZIP family.,similarity:Belongs to the bZIP family. Fos subfamily.,similarity:Contains 1 bZIP domain.,subunit:Heterodimer.,
- 组织表达：
- [Isoform 11]: Expressed in the nucleus accumbens of the striatum (at protein level).
- Western Blot analysis of various cells using Fos B Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunofluorescence analysis of HepG2 cells, using FosB Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from COS7 and Jurkat cells, using FosB Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
