Fli-1 Polyclonal Antibody
- 货号：YT1720
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse
- 简介：
- >>Transcriptional misregulation in cancer
- 蛋白名称：
- Friend leukemia integration 1 transcription factor
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human FLI1. AA range:291-340
- 特异性：
- Fli-1 Polyclonal Antibody detects endogenous levels of Fli-1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- FLI1;Friend leukemia integration 1 transcription factor;Proto-oncogene Fli-1;Transcription factor ERGB
- 背景：
- This gene encodes a transcription factor containing an ETS DNA-binding domain. The gene can undergo a t(11;22)(q24;q12) translocation with the Ewing sarcoma gene on chromosome 22, which results in a fusion gene that is present in the majority of Ewing sarcoma cases. An acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation involving this gene has also been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012],
- 功能：
- disease:A chromosomal aberration involving FLI1 is a cause of Ewing sarcoma [MIM:133450]. Translocation t(11;22)(q24;q12) with EWS.,function:Sequence-specific transcriptional activator. Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.,miscellaneous:Located on a fragment of chromosome 11 flanked on the centromeric side by the acute lymphoblastic leukemia-associated t(4;11)(q21;q23) translocation breakpoint and on the telomeric side by the Ewing- and neuroepithelioma-associated t(11;22) (q24;q12) breakpoint.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,similarity:Contains 1 PNT (pointed) domain.,subunit:Can form homodimers or heterodimers with ETV6/TEL1.,
- 组织表达：
- Blood,Bone marrow,Lymph,Placenta,
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- Western Blot analysis of 3T3 cells using Fli-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FLI1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from NIH/3T3 cells, using FLI1 Antibody. The lane on the right is blocked with the synthesized peptide.
