FAST-1/2 Polyclonal Antibody
- 货号:YT1679
- 应用:WB;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Forkhead box protein H1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human FOXH1. AA range:10-59
- 特异性:
- FAST-1/2 Polyclonal Antibody detects endogenous levels of FAST-1/2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- FOXH1;FAST1;FAST2;Forkhead box protein H1;Forkhead activin signal transducer 1;Fast-1;hFAST-1;Forkhead activin signal transducer 2;Fast-2
- 背景:
- FOXH1 encodes a human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 protein binds SMAD2 and activates an activin response element via binding the DNA motif TGT(G/T)(T/G)ATT. [provided by RefSeq, Jul 2008],
- 功能:
- domain:The FM region is required for binding smad2/smad4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated smad2 that is in an activated smad complex.,function:Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).,similarity:Contains 1 fork-head DNA-binding domain.,subunit:Interacts with the MH2 domains of SMAD2 and SMAD3.,tissue specificity:Ubiquitous.,
![](/uploadfiles/PIC/yt1679-wb.jpg)
- Western Blot analysis of various cells using FAST-1/2 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
![](/uploadfiles/PIC/yt1679_pic492118522.jpg)
- Immunofluorescence analysis of A549 cells, using FOXH1 Antibody. The picture on the right is blocked with the synthesized peptide.