ERCC4 Polyclonal Antibody
- 货号：YT1615
- 应用：WB;ELISA
- 种属：Human;Mouse
- 简介：
- >>Nucleotide excision repair;>>Fanconi anemia pathway
- 蛋白名称：
- DNA repair endonuclease XPF
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human XPF. AA range:801-850
- 特异性：
- ERCC4 Polyclonal Antibody detects endogenous levels of ERCC4 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ERCC4;ERCC11;XPF;DNA repair endonuclease XPF;DNA excision repair protein ERCC-4;DNA repair protein complementing XP-F cells;Xeroderma pigmentosum group F-complementing protein
- 背景：
- The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).[provided by RefSeq, Mar 2009],
- 功能：
- cofactor:Magnesium.,disease:Defects in ERCC4 are a cause of XFE progeroid syndrome [MIM:610965]. This syndrome is illustrated by one patient who presented with dwarfism, cachexia and microcephaly.,disease:Defects in ERCC4 are the cause of xeroderma pigmentosum complementation group F (XP-F) [MIM:278760]; also known as xeroderma pigmentosum VI (XP6). XP-F is an autosomal recessive disease characterized by hypersensitivity of the skin to sunlight followed by high incidence of skin cancer and frequent neurologic abnormalities.,function:Structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link.,similarity:Belongs to the XPF family.,subunit:Heterodimer composed of ERCC1 and XPF/ERCC4. Interacts with EME1.,
- 细胞定位：
- Nucleus . Chromosome . Localizes to sites of DNA damage. .
- 组织表达：
- Epithelium,Fibroblast,
- Western Blot analysis of various cells using ERCC4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of 293 cells using ERCC4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from 293 cells, using XPF Antibody. The lane on the right is blocked with the synthesized peptide.
