EphB1/2 Polyclonal Antibody
- 货号:YT1583
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Ephrin type-B receptor 1/2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human EPHB1/2. AA range:561-610
- 特异性:
- EphB1/2 Polyclonal Antibody detects endogenous levels of EphB1/2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- EPHB1;ELK;EPHT2;HEK6;NET;Ephrin type-B receptor 1;ELK;EPH tyrosine kinase 2;EPH-like kinase 6;EK6;hEK6;Neuronally-expressed EPH-related tyrosine kinase;NET;Tyrosine-protein kinase receptor EPH-2;EPHB2;DRT;EPHT3;EPTH3;ERK;
- 背景:
- Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. May be involved in cell-cell interactions in the nervous system.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,subunit:The ligand-activated form interacts with GRB2, GRB10 and NCK through their respective SH2 domains. The GRB10 SH2 domain binds EPHB1 through Tyr-928, while GRB2 binds residues within the catalytic domain. Interacts with EPHB6. The NCK SH2 domain binds EPHB1 through Tyr-594. Interacts with PRKCABP.,tissue specificity:Preferentially expressed in brain.,
- 细胞定位:
- Cell membrane ; Single-pass type I membrane protein . Early endosome membrane . Cell projection, dendrite .
- 组织表达:
- Preferentially expressed in brain.
![](/uploadfiles/PIC/yt1583-wb.jpg)
- Western Blot analysis of various cells using EphB1/2 Polyclonal Antibody diluted at 1:500
![](/uploadfiles/PIC/yt1583-wb(2).jpg)
- Western Blot analysis of HepG2 cells using EphB1/2 Polyclonal Antibody diluted at 1:500
![](/uploadfiles/PIC/yt1583_pic325818522.jpg)
- Immunofluorescence analysis of HUVEC cells, using EPHB1/2 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1583_pic325918522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EPHB1/2 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1583_pic326018522.jpg)
- Western blot analysis of lysates from HepG2 cells, using EPHB1/2 Antibody. The lane on the right is blocked with the synthesized peptide.