EphA1 Polyclonal Antibody
- 货号：YT1573
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- Ephrin type-A receptor 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human EPHA1. AA range:541-590
- 特异性：
- EphA1 Polyclonal Antibody detects endogenous levels of EphA1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- EPHA1;EPH;EPHT;EPHT1;Ephrin type-A receptor 1;hEpha1;EPH tyrosine kinase;EPH tyrosine kinase 1;Erythropoietin-producing hepatoma receptor;Tyrosine-protein kinase receptor EPH
- 背景：
- This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,function:Receptor for members of the ephrin-A family. Binds with a low affinity to ephrin-A1.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 fibronectin type-III domains.,tissue specificity:Overexpressed in several carcinomas.,
- 细胞定位：
- Cell membrane ; Single-pass type I membrane protein .
- 组织表达：
- Overexpressed in several carcinomas.
- Western Blot analysis of various cells using EphA1 Polyclonal Antibody
- Immunofluorescence analysis of HeLa cells, using EPHA1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from COLO205 cells, using EPHA1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
