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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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EMR1 Polyclonal Antibody
- 货号:YT1544
- 应用:WB;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- EGF-like module-containing mucin-like hormone receptor-like 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human EMR1. AA range:818-867
- 特异性:
- EMR1 Polyclonal Antibody detects endogenous levels of EMR1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- EMR1;TM7LN3;EGF-like module-containing mucin-like hormone receptor-like 1;EGF-like module receptor 1;EMR1 hormone receptor
- 背景:
- This gene encodes a protein that has a domain resembling seven transmembrane G protein-coupled hormone receptors (7TM receptors) at its C-terminus. The N-terminus of the encoded protein has six EGF-like modules, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],
- 功能:
- alternative products:Comment=Additional isoforms seem to exist,function:Could be involved in cell-cell interactions.,similarity:Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.,similarity:Contains 1 GPS domain.,similarity:Contains 6 EGF-like domains.,tissue specificity:Wide expression; increased levels in peripheral blood mononuclear cells.,
- 细胞定位:
- Cell membrane ; Multi-pass membrane protein .
- 组织表达:
- Expression is restricted to eosinophils.
- Western Blot analysis of Jurkat cells using EMR1 Polyclonal Antibody
- Western blot analysis of lysates from Jurkat cells, using EMR1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using EMR1 antibody.