Elongin A1 Polyclonal Antibody
- 货号:YT1532
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Transcription elongation factor B polypeptide 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human TCEB3. AA range:131-180
- 特异性:
- Elongin A1 Polyclonal Antibody detects endogenous levels of Elongin A1 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- TCEB3;MSTP059;Transcription elongation factor B polypeptide 3;Elongin 110 kDa subunit;Elongin-A;EloA;RNA polymerase II transcription factor SIII subunit A1;SIII p110
- 背景:
- This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. [provided by RefSeq, Jul 2008],
- 功能:
- domain:The elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV].,function:SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).,similarity:Contains 1 F-box domain.,similarity:Contains 1 TFIIS N-terminal domain.,subunit:Heterotrimer of an A (A1, A2 or A3), B and C subunit. The C subunit mediates the binding of the elongin BC complex to subunit A.,
- 组织表达:
- Epithelium,Heart,Lung,Umbilical vein,
![](/uploadfiles/PIC/yt1532-wb.jpg)
- Western Blot analysis of various cells using Elongin A1 Polyclonal Antibody
![](/uploadfiles/PIC/yt1532_pic627018522.jpg)
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ELOA1 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1532_pic627118522.jpg)
- Western blot analysis of lysates from HUVEC cells and Jurkat cells, using ELOA1 Antibody. The lane on the right is blocked with the synthesized peptide.