EKLF/CKLF/UKLF Polyclonal Antibody
- 货号：YT1522
- 应用：WB;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Krueppel-like factor 1/Krueppel-like factor 5/Krueppel-like factor 7
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human KLF. AA range:291-340
- 特异性：
- EKLF/CKLF/UKLF Polyclonal Antibody detects endogenous levels of EKLF/CKLF/UKLF protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- KLF1;EKLF;Krueppel-like factor 1;Erythroid krueppel-like transcription factor;EKLF;KLF5;BTEB2;CKLF;IKLF;Krueppel-like factor 5;Basic transcription element-binding protein 2;BTE-binding protein 2;Colon krueppel-like factor;GC-bo
- 实测条带：
- 38kD(EKLF) 20kD(CKLF/UKLF)
- 背景：
- This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype. [provided by RefSeq, Oct 2009],
- 功能：
- function:Transcription regulator of erythrocyte development. Binds to the CACCC box in the beta-globin gene promoter and activates transcription. When sumoylation, acts as a Probably serves as a general switch factor for erythroid development. When sumoylated, acts as a transcriptional repressor, by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.,PTM:Acetylated; can be acetylated on both Lys-274 and Lys-288. Acetylation on Lys-274 (by CBP) appears to be the major site affecting EKLF transactivation activity.,PTM:Phosphorylated primarily on serine residues in the transactivation domain. Phosphorylation on Thr-23 is critical for the transactivation activity.,PTM:Sumoylated; sumoylation, promoted by PIAS1, leads to repression of megakaryocyte differentiation. Also promotes the interaction with the CDH4 subunit of the NuRD repression complex.,simila
- 细胞定位：
- Nucleus . Colocalizes with SUMO1 in nuclear speckles. .
- 组织表达：
- Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines.
- Western Blot analysis of various cells using EKLF/CKLF/UKLF Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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- Western Blot analysis of VEC cells using EKLF/CKLF/UKLF Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from Jurkat cells, treated with serum 20% 15', using KLF Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from K562 cells using KLF antibody.
