E4BP4 Polyclonal Antibody
- 货号：YT1447
- 应用：WB;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Nuclear factor interleukin-3-regulated protein
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human NFIL3. AA range:61-110
- 特异性：
- E4BP4 Polyclonal Antibody detects endogenous levels of E4BP4 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NFIL3;E4BP4;IL3BP1;Nuclear factor interleukin-3-regulated protein;E4 promoter-binding protein 4;Interleukin-3 promoter transcriptional activator;Interleukin-3-binding protein 1;Transcriptional activator NF-IL3A
- 背景：
- The protein encoded by this gene is a transcriptional regulator that binds as a homodimer to activating transcription factor (ATF) sites in many cellular and viral promoters. The encoded protein represses PER1 and PER2 expression and therefore plays a role in the regulation of circadian rhythm. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2014],
- 功能：
- function:Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts (By similarity). Represses transcriptional activity of PER1 (By similarity). Represses transcriptional activity of PER2 via the B-site on the promoter (By similarity). Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF) (By similarity). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock (By similarity). Protects pro-B cells from programmed cell death.,induction:Up-regulated by PHA or TP
- 组织表达：
- Expressed in bladder stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and muscle.
- Western Blot analysis of various cells using E4BP4 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Western blot analysis of lysates from 293 and K562 cells, using NFIL3 Antibody. The lane on the right is blocked with the synthesized peptide.
