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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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DPF2 Polyclonal Antibody
- 货号:YT1408
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 蛋白名称:
- Zinc finger protein ubi-d4
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human REQU. AA range:151-200
- 特异性:
- DPF2 Polyclonal Antibody detects endogenous levels of DPF2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DPF2;BAF45D;REQ;UBID4;Zinc finger protein ubi-d4;Apoptosis response zinc finger protein;BRG1-associated factor 45D;BAF45D;D4; zinc and double PHD fingers family 2;Protein requiem
- 背景:
- The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008],
- 功能:
- function:May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells.,similarity:Belongs to the requiem/DPF family.,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 2 PHD-type zinc fingers.,subcellular location:30% nuclear. 70% cytoplasmic.,tissue specificity:Ubiquitous.,
- 细胞定位:
- Nucleus . Cytoplasm .
- Western Blot analysis of various cells using DPF2 Polyclonal Antibody
- Immunofluorescence analysis of A549 cells, using REQU Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HT-29 cells, using REQU Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from RAW264.7cells using REQU antibody.