Dok-3 Polyclonal Antibody
- 货号:YT1397
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human DOK3. AA range:101-150
- 特异性:
- Dok-3 Polyclonal Antibody detects endogenous levels of Dok-3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DOK3;Docking protein 3;Downstream of tyrosine kinase 3
- 背景:
- domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL function.,PTM:Constitutively tyrosine-phosphorylated.,PTM:On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.,similarity:Belongs to the DOK family. Type A subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain and in a kinase-dependent manner. Does not interact with RasGAP.,tissue specificity:Expressed in spleen.,
- 功能:
- domain:PTB domain mediates receptor interaction.,function:DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL function.,PTM:Constitutively tyrosine-phosphorylated.,PTM:On IL2 stimulation, phosphorylated on C-terminal tyrosine residues possibly by Src kinases. Can also be phosphorylated by ABL kinase.,similarity:Belongs to the DOK family. Type A subfamily.,similarity:Contains 1 IRS-type PTB domain.,similarity:Contains 1 PH domain.,subunit:On tyrosine phosphorylation, interacts with CSK and INPP5D/SHIP1 via their SH2 domains. Both Tyr-381 and Tyr-398 are required for interaction with INPP5D. Only Tyr-381 is required for interaction with CSK. Binds ABL through the PTB domain
- 细胞定位:
- Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side .
- 组织表达:
- Expressed in spleen.
![](/uploadfiles/PIC/yt1397-wb.jpg)
- Western Blot analysis of various cells using Dok-3 Polyclonal Antibody
![](/uploadfiles/PIC/yt1397_pic555718522.jpg)
- Western blot analysis of lysates from HepG2 cells, using DOK3 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1397_pic981318522.jpg)
- Western blot analysis of the lysates from K562 cells using DOK3 antibody.
![](/uploadfiles/PIC/46-ihc-xiaochangaic20220712.jpg)
- Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1,primary Antibody was diluted at 1:200(4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200