DNAM-1 Polyclonal Antibody
- 货号：YT1381
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Monkey
- 简介：
- >>Cell adhesion molecules
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human CD226/DNAM-1. AA range:287-336
- 特异性：
- DNAM-1 Polyclonal Antibody detects endogenous levels of DNAM-1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CD226;DNAM1;CD226 antigen;DNAX accessory molecule 1;DNAM-1;CD antigen CD226
- 背景：
- This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015],
- 功能：
- function:Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.,PTM:Phosphorylated.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,subunit:Interacts with PVR and PVRL2.,tissue specificity:Expressed by peripheral blood T-lymphocytes.,
- 细胞定位：
- Cell membrane ; Single-pass type I membrane protein .
- 组织表达：
- Expressed by peripheral blood T-lymphocytes.
.jpg)
- Western Blot analysis of COS7 cells using DNAM-1 Polyclonal Antibody diluted at 1:500
- Western Blot analysis of mouse-brain cells using DNAM-1 Polyclonal Antibody diluted at 1:500
- Western Blot analysis of AD293 using DNAM-1 Polyclonal Antibody diluted at 1:500
- Immunofluorescence analysis of HeLa cells, using CD226/DNAM-1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from COS7 and K562 cells, treated with PMA 125ng/ml 30' , using CD226/DNAM-1 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HUVECcells using CD226/DNAM-1 antibody.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
