• 靶点：
• DNA pol β

• 简介：
• >>Base excision repair;>>Human T-cell leukemia virus 1 infection;>>Viral carcinogenesis

• 基因名称：
• POLB

• 蛋白名称：
• DNA polymerase beta

• Human Gene Id：
• 5423

• Human Swiss Prot No：
• P06746

• Mouse Gene Id：
• 18970

• Mouse Swiss Prot No：
• Q8K409

• Rat Gene Id：
• 29240

• Rat Swiss Prot No：
• P06766

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human DNA Polymerase beta. AA range:286-335

• 特异性：
• DNA pol β Polyclonal Antibody detects endogenous levels of DNA pol β protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• POLB;DNA polymerase beta

• 实测条带：
• 38kD

• 背景：
• The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011],

• 功能：
• catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,cofactor:Binds 2 magnesium ions per subunit.,domain:Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.,function:Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.,PTM:Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.,similarity:Belongs to the DNA polymerase type-X family.,subunit:Monomer.,

• 细胞定位：
• Nucleus. Cytoplasm. Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.

• 组织表达：
• Skin,Testis,

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