DNA pol α Polyclonal Antibody
- 货号：YT1368
- 应用：WB;ELISA
- 种属：Human;Rat;Mouse;
- 蛋白名称：
- DNA polymerase alpha catalytic subunit
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human DNA Polymerase alpha. AA range:81-130
- 特异性：
- DNA pol α Polyclonal Antibody detects endogenous levels of DNA pol α protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- POLA1;POLA;DNA polymerase alpha catalytic subunit;DNA polymerase alpha catalytic subunit p180
- 背景：
- This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010],
- 功能：
- catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Polymerase alpha in a complex with DNA primase is a replicative polymerase.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,PTM:A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.,similarity:Belongs to the DNA polymerase type-B family.,subunit:Interacts with SV40 Large T antigen; this interaction allows viral DNA replication.,
- 细胞定位：
- Nucleus . Cytoplasm, cytosol . In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern. .
- Western Blot analysis of various cells using DNA pol α Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from HepG2, 293, and Jurkat cells, using DNA Polymerase alpha Antibody. The lane on the right is blocked with the synthesized peptide.
