DNA Ligase I Polyclonal Antibody
- 货号：YT1364
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>DNA replication;>>Base excision repair;>>Nucleotide excision repair;>>Mismatch repair
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human DNL1. AA range:111-160
- 特异性：
- DNA Ligase I Polyclonal Antibody detects endogenous levels of DNA Ligase I protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- LIG1;DNA ligase 1;DNA ligase I;Polydeoxyribonucleotide synthase [ATP] 1
- 背景：
- This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
- 功能：
- catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG1 seem to cause immunodeficiencies and cellular hypersensitivity to DNA-damaging agents.,function:DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.,online information:DNA ligase entry,online information:LIG1 mutation db,similarity:Belongs to the ATP-dependent DNA ligase family.,
- 组织表达：
- Brain,Epithelium,Eye,PCR rescued clones,T lymphoblast,
- Western Blot analysis of various cells using DNA Ligase I Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using DNL1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HT-29 cells, using DNL1 Antibody. The lane on the right is blocked with the synthesized peptide.
