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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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DIO3 Polyclonal Antibody
- 货号:YT1352
- 应用:WB;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Thyroid hormone signaling pathway
- 蛋白名称:
- Type III iodothyronine deiodinase
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human DIO3. AA range:17-66
- 特异性:
- DIO3 Polyclonal Antibody detects endogenous levels of DIO3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DIO3;ITDI3;TXDI3;Type III iodothyronine deiodinase;5DIII;DIOIII;Type 3 DI;Type-III 5'-deiodinase
- 背景:
- The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protei
- 功能:
- catalytic activity:3,3',5'-triiodo-L-thyronine + iodide + A + H(+) = L-thyroxine + AH(2).,function:Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development.,function:Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine).,similarity:Belongs to the iodothyronine deiodinase family.,tissue specificity:Expressed in placenta and several fetal tissues.,
- 细胞定位:
- Cell membrane ; Single-pass type II membrane protein . Endosome membrane ; Single-pass type II membrane protein .
- 组织表达:
- Expressed in placenta and several fetal tissues.
![](/uploadfiles/PIC/yt1352-wb.jpg)
- Western Blot analysis of various cells using DIO3 Polyclonal Antibody
![](/uploadfiles/PIC/yt1352_pic926518522.jpg)
- Western blot analysis of lysate from HUVEC cells, using DIO3 antibody.