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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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DHS Polyclonal Antibody
- 货号:YT1350
- 应用:WB;ELISA
- 种属:Human;Rat;Mouse;
- 蛋白名称:
- Deoxyhypusine synthase
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human DHPS. AA range:51-100
- 特异性:
- DHS Polyclonal Antibody detects endogenous levels of DHS protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DHPS;DS;Deoxyhypusine synthase;DHS
- 背景:
- This gene encodes a protein that is required for the formation of hypusine, a unique amino acid formed by the posttranslational modification of only one protein, eukaryotic translation initiation factor 5A. The encoded protein catalyzes the first step in hypusine formation by transferring the butylamine moiety of spermidine to a specific lysine residue of the eukaryotic translation initiation factor 5A precursor, forming an intermediate deoxyhypusine residue. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2011],
- 功能:
- catalytic activity:[eIF5A-precursor]-lysine + spermidine = [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine.,cofactor:NAD.,function:Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.,pathway:Protein modification; eIF5A hypusination.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the deoxyhypusine synthase family.,subunit:Homotetramer formed by a dimer of dimers.,
- 组织表达:
- Blood,Brain,Cervix carcinoma,Lung,
![](/uploadfiles/PIC/yt1350-wb.jpg)
- Western Blot analysis of various cells using DHS Polyclonal Antibody
![](/uploadfiles/PIC/yt1350-wb(2).jpg)
- Western Blot analysis of K562 cells using DHS Polyclonal Antibody
![](/uploadfiles/PIC/yt1350_pic685018522.jpg)
- Western blot analysis of lysates from NIH/3T3 cells, using DHPS Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1350_pic979818522.jpg)
- Western blot analysis of the lysates from HUVECcells using DHPS antibody.