DGK-ζ Polyclonal Antibody
- 货号：YT1335
- 应用：IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Choline metabolism in cancer
- 蛋白名称：
- Diacylglycerol kinase zeta
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human DGKZ. AA range:601-650
- 特异性：
- DGK-ζ Polyclonal Antibody detects endogenous levels of DGK-ζ protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- DGKZ;DAGK6;Diacylglycerol kinase zeta;DAG kinase zeta;Diglyceride kinase zeta;DGK-zeta
- 背景：
- The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and multiple transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2010],
- 功能：
- catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.,PTM:Phosphorylation of the MARCKS homology domain by PKC reduces nuclear accumulation of DGK-zeta.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 ANK repeats.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:Interacts with the PDZ domain of the syntrophin SNTG1 and that of SNX27. Isoform 2 forms a signaling complex with RASGRP1 and HRAS.,tissue speci
- 细胞定位：
- Nucleus . Cytoplasm, cytosol . Cell membrane . Cell projection, lamellipodium .
- 组织表达：
- Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. ; [Isoform 2]: Predominantly expressed in muscle.
- Immunohistochemistry analysis of paraffin-embedded human brain, using DGKZ Antibody. The picture on the right is blocked with the synthesized peptide.
