DGK-ε Polyclonal Antibody
- 货号：YT1334
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Choline metabolism in cancer
- 蛋白名称：
- Diacylglycerol kinase epsilon
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human DGKE. AA range:161-210
- 特异性：
- DGK-ε Polyclonal Antibody detects endogenous levels of DGK-ε protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- DGKE;DAGK5;Diacylglycerol kinase epsilon;DAG kinase epsilon;Diglyceride kinase epsilon;DGK-epsilon
- 背景：
- Diacylglycerol kinases are thought to be involved mainly in the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. When expressed in mammalian cells, DGK-epsilon shows specificity for arachidonyl-containing diacylglycerol. DGK-epsilon is expressed predominantly in testis. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,function:Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Expressed predominantly in testis.,
- 细胞定位：
- Membrane ; Single-pass membrane protein . Cytoplasm .
- 组织表达：
- Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
- Western blot analysis of lysates from K562 cells, using DGKE Antibody. The lane on the right is blocked with the synthesized peptide.
