DGK-δ Polyclonal Antibody
- 货号：YT1333
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Glycerolipid metabolism;>>Glycerophospholipid metabolism;>>Metabolic pathways;>>Phosphatidylinositol signaling system;>>Phospholipase D signaling pathway;>>Choline metabolism in cancer
- 蛋白名称：
- Diacylglycerol kinase delta
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human DGKD. AA range:41-90
- 特异性：
- DGK-δ Polyclonal Antibody detects endogenous levels of DGK-δ protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- DGKD;KIAA0145;Diacylglycerol kinase delta;DAG kinase delta;130 kDa diacylglycerol kinase;Diglyceride kinase delta;DGK-delta
- 背景：
- This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.,enzyme regulation:Partially inhibited by phosphatidylserine.,function:May function as signaling molecule. Isoform 2 may be involved in cell growth and tumorigenesis.,PTM:Isoform 1 H domain is phosphorylated.,similarity:Belongs to the eukaryotic diacylglycerol kinase family.,similarity:Contains 1 DAGKc domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,subunit:The two isoforms are able to form homo- and hetero-oligomer structures (at least tetramers).,tissue specificity:Isoform 2 is ubiquitously expressed also in tumor tissues. Isoform 1 is expressed in ovary, spleen and some tumor-derived cells.,
- 细胞定位：
- Membrane, clathrin-coated pit . Cytoplasm .; [Isoform 1]: Cell membrane ; Peripheral membrane protein . Cytoplasm . Isoform 1 translocation from cytoplasm to the plasma membrane is induced by phorbol esters (PubMed:12200442). Phorbol esters induce the conversion into the monomeric form which can translocate to the plasma membrane (PubMed:12084710). .
- 组织表达：
- [Isoform 2]: Widely expressed. ; [Isoform 1]: Only detected in ovary, and to a lesser extent in spleen.
- Western Blot analysis of various cells using DGK-δ Polyclonal Antibody
- Western blot analysis of lysates from HUVEC cells, using DGKD Antibody. The lane on the right is blocked with the synthesized peptide.
