• 靶点：
• Cytokeratin 20

• 简介：
• >>Estrogen signaling pathway;>>Staphylococcus aureus infection

• 基因名称：
• KRT20

• 蛋白名称：
• Keratin type I cytoskeletal 20

• Human Gene Id：
• 54474

• Human Swiss Prot No：
• P35900

• Mouse Gene Id：
• 66809

• Mouse Swiss Prot No：
• Q9D312

• Rat Gene Id：
• 286912

• Rat Swiss Prot No：
• P25030

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human Keratin 20. AA range:375-424

• 特异性：
• Cytokeratin 20 Polyclonal Antibody detects endogenous levels of Cytokeratin 20 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• KRT20;Keratin; type I cytoskeletal 20;Cytokeratin-20;CK-20;Keratin-20;K20;Protein IT

• 实测条带：
• 49kD

• 背景：
• The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008],

• 功能：
• developmental stage:First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.,function:Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during t

• 细胞定位：
• Cytoplasm .

• 组织表达：
• Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

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