Cytochrome b5 Polyclonal Antibody
- 货号:YT1257
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110
- 特异性:
- Cytochrome b5 Polyclonal Antibody detects endogenous levels of Cytochrome b5 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CYB5A;CYB5;Cytochrome b5;Microsomal cytochrome b5 type A;MCB5
- 背景:
- The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010],
- 功能:
- disease:Defects in CYB5A are the cause of type IV hereditary methemoglobinemia [MIM:250790].,function:Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.,similarity:Belongs to the cytochrome b5 family.,similarity:Contains 1 cytochrome b5 heme-binding domain.,
- 细胞定位:
- [Isoform 1]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm.
- 组织表达:
- Erythrocyte,Liver,Spleen,Uterus,
![](/uploadfiles/PIC/yt1257-wb.jpg)
- Western Blot analysis of various cells using Cytochrome b5 Polyclonal Antibody
![](/uploadfiles/PIC/yt1257_pic625818522.jpg)
- Immunofluorescence analysis of NIH/3T3 cells, using CYB5 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt1257_pic625918522.jpg)
- Western blot analysis of lysates from A549 cells, using CYB5 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/11-ihc-ganaicm202207.jpg)
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).