• 靶点：
• CYP4F2

• 简介：
• >>Arachidonic acid metabolism;>>Metabolic pathways

• 基因名称：
• CYP4F2

• 蛋白名称：
• Leukotriene-B(4) omega-hydroxylase 1

• Human Gene Id：
• 8529

• Human Swiss Prot No：
• P78329

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human CYP4F2. AA range:41-90

• 特异性：
• CYP4F2 Polyclonal Antibody detects endogenous levels of CYP4F2 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• CYP4F2;Leukotriene-B(4) omega-hydroxylase 1;CYPIVF2;Cytochrome P450 4F2;Cytochrome P450-LTB-omega;Leukotriene-B(4) 20-monooxygenase 1

• 实测条带：
• 60kD

• 背景：
• This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O.,cofactor:Heme group.,function:Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.,similarity:Belongs to the cytochrome P450 family.,tissue specificity:Liver.,

• 细胞定位：
• Microsome membrane ; Peripheral membrane protein . Endoplasmic reticulum membrane ; Peripheral membrane protein .

• 组织表达：
• Liver. Also present in kidney: specifically expressed in the S2 and S3 segments of proximal tubules in cortex and outer medulla (PubMed:10660572).

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