CRABP-II Polyclonal Antibody
- 货号：YT1094
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Cellular retinoic acid-binding protein 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human CRABP2. AA range:41-90
- 特异性：
- CRABP-II Polyclonal Antibody detects endogenous levels of CRABP-II protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CRABP2;Cellular retinoic acid-binding protein 2;Cellular retinoic acid-binding protein II;CRABP-II
- 背景：
- This gene encodes a member of the retinoic acid (RA, a form of vitamin A) binding protein family and lipocalin/cytosolic fatty-acid binding protein family. The protein is a cytosol-to-nuclear shuttling protein, which facilitates RA binding to its cognate receptor complex and transfer to the nucleus. It is involved in the retinoid signaling pathway, and is associated with increased circulating low-density lipoprotein cholesterol. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Dec 2010],
- 功能：
- domain:Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.,function:Transports retinoic acid to the nucleus. Regulates the access of retinoic acid to the nuclear retinoic acid receptors.,induction:By retinoic acid.,similarity:Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.,subcellular location:Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.,subunit:Interacts with RXR and RARA (By similarity). Interacts with importin alpha.,
- 细胞定位：
- Cytoplasm. Endoplasmic reticulum. Nucleus. Upon ligand binding, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus.
- Western Blot analysis of various cells using CRABP-II Polyclonal Antibody
- Western blot analysis of lysates from HT-29 cells, using CRABP2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
