COX4I2 Polyclonal Antibody
- 货号：YT1075
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>Oxidative phosphorylation;>>Metabolic pathways;>>Cardiac muscle contraction;>>Thermogenesis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Huntington disease;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Chemical carcinogenesis - reactive oxygen species;>>Diabetic cardiomyopathy
- 蛋白名称：
- Cytochrome c oxidase subunit 4 isoform 2 mitochondrial
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human COX42. AA range:31-80
- 特异性：
- COX4I2 Polyclonal Antibody detects endogenous levels of COX4I2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- COX4I2;COX4L2;Cytochrome c oxidase subunit 4 isoform 2; mitochondrial;Cytochrome c oxidase subunit IV isoform 2;COX IV-2
- 背景：
- Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes isoform 2 of subunit IV. Isoform 1 of subunit IV is encoded by a different gene, however, the two genes show a similar structural organization. Subunit IV is the largest nuclear encoded subunit which plays a pivotal role in COX regulation. [provided by RefSeq, Jul 2008],
- 功能：
- function:This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.,similarity:Belongs to the cytochrome c oxidase IV family.,tissue specificity:Highly expressed in lung.,
- 细胞定位：
- Mitochondrion inner membrane ; Single-pass membrane protein .
- 组织表达：
- Highly expressed in lung.
- Western Blot analysis of K562 insulin 0.01U/ml 15' cells using COX4I2 Polyclonal Antibody
- Immunofluorescence analysis of COS7 cells, using COX42 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from K562 cells, treated with insulin 0.01U/ml 15', using COX42 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
