Cox-1 Polyclonal Antibody
- 货号：YT1067
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Rat;Mouse;
- 简介：
- >>Arachidonic acid metabolism;>>Metabolic pathways;>>Platelet activation;>>Serotonergic synapse;>>Regulation of lipolysis in adipocytes
- 蛋白名称：
- Prostaglandin G/H synthase 1
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human Cox1. AA range:550-599
- 特异性：
- Cox-1 Polyclonal Antibody detects endogenous levels of Cox-1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PTGS1;COX1;Prostaglandin G/H synthase 1;Cyclooxygenase-1;COX-1;Prostaglandin H2 synthase 1;PGH synthase 1;PGHS-1;PHS 1;Prostaglandin-endoperoxide synthase 1
- 背景：
- This is one of two genes encoding similar enzymes that catalyze the conversion of arachinodate to prostaglandin. The encoded protein regulates angiogenesis in endothelial cells, and is inhibited by nonsteroidal anti-inflammatory drugs such as aspirin. Based on its ability to function as both a cyclooxygenase and as a peroxidase, the encoded protein has been identified as a moonlighting protein. The protein may promote cell proliferation during tumor progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014],
- 功能：
- catalytic activity:Arachidonate + AH(2) + 2 O(2) = prostaglandin H(2) + A + H(2)O.,cofactor:Binds 1 heme B (iron-protoporphyrin IX) group per subunit.,function:May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.,miscellaneous:This enzyme acts both as a dioxygenase and as a peroxidase.,miscellaneous:This enzyme is the target of nonsteroidal anti-inflammatory drugs such as aspirin.,pathway:Lipid metabolism; prostaglandin biosynthesis.,similarity:Belongs to the prostaglandin G/H synthase family.,similarity:Contains 1 EGF-like domain.,subunit:Homodimer.,
- 细胞定位：
- Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
- 组织表达：
- Brain,Lung fibroblast,Platelet,
- Western Blot analysis of various cells using Cox-1 Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Cox1 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from HUVEC cells, using Cox1 Antibody. The lane on the right is blocked with the synthesized peptide.
