Connexin 25 Polyclonal Antibody
- 货号：YT1041
- 应用：WB;ELISA
- 种属：Human
- 蛋白名称：
- Gap junction beta-7 protein
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human GJB7. AA range:21-70
- 特异性：
- Connexin 25 Polyclonal Antibody detects endogenous levels of Connexin 25 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- GJB7;CX25;Gap junction beta-7 protein;Connexin-25;Cx25
- 背景：
- Connexins, such as GJB7, are involved in the formation of gap junctions, intercellular conduits that directly connect the cytoplasms of contacting cells. Each gap junction channel is formed by docking of 2 hemichannels, each of which contains 6 connexin subunits (Sohl et al., 2003 [PubMed 12881038]).[supplied by OMIM, Mar 2008],
- 功能：
- function:One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.,similarity:Belongs to the connexin family. Beta-type (group I) subfamily.,subunit:A connexon is composed of a hexamer of connexins.,tissue specificity:Weakly expressed in placenta.,
- 细胞定位：
- Cell membrane ; Multi-pass membrane protein . Cell junction, gap junction .
- 组织表达：
- Weakly expressed in placenta.
- Western Blot analysis of various cells using Connexin 25 Polyclonal Antibody diluted at 1:500
- Western blot analysis of lysates from HT-29 cells, using GJB7 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HeLa cells using GJB7 antibody.
