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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CNT2 Polyclonal Antibody
- 货号:YT1004
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Sodium/nucleoside cotransporter 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human SLC28A2. AA range:371-420
- 特异性:
- CNT2 Polyclonal Antibody detects endogenous levels of CNT2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- SLC28A2;CNT2;Sodium/nucleoside cotransporter 2;Concentrative nucleoside transporter 2;CNT 2;hCNT2;Na(+)/nucleoside cotransporter 2;Sodium-coupled nucleoside transporter 2;Sodium/purine nucleoside co-transporter;SPNT;Solute carrier
- 背景:
- enzyme regulation:Inhibited by formycin B.,function:Sodium-dependent and purine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues.,similarity:Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.,tissue specificity:Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain. Weak expression in lung.,
- 功能:
- enzyme regulation:Inhibited by formycin B.,function:Sodium-dependent and purine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues.,similarity:Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.,tissue specificity:Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain. Weak expression in lung.,
- 细胞定位:
- Membrane ; Multi-pass membrane protein .
- 组织表达:
- Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain (PubMed:9435697). Weak expression in lung (PubMed:9435697).
- Western Blot analysis of various cells using CNT2 Polyclonal Antibody
- Western Blot analysis of 3T3 cells using CNT2 Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using SLC28A2 Antibody. The picture on the right is blocked with the synthesized peptide.
- Western blot analysis of lysates from Jurkat cells, using SLC28A2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Western blot analysis of the lysates from HepG2 cells using SLC28A2 antibody.