CLIP3 Polyclonal Antibody
- 货号:YT0969
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- CAP-Gly domain-containing linker protein 3
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CLIP3. AA range:361-410
- 特异性:
- CLIP3 Polyclonal Antibody detects endogenous levels of CLIP3 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CLIP3;CLIPR59;CAP-Gly domain-containing linker protein 3;Cytoplasmic linker protein 170-related 59 kDa protein;CLIP-170-related 59 kDa protein;CLIPR-59
- 背景:
- This gene encodes a member of the cytoplasmic linker protein 170 family. Members of this protein family contain a cytoskeleton-associated protein glycine-rich domain and mediate the interaction of microtubules with cellular organelles. The encoded protein plays a role in T cell apoptosis by facilitating the association of tubulin and the lipid raft ganglioside GD3. The encoded protein also functions as a scaffold protein mediating membrane localization of phosphorylated protein kinase B. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010],
- 功能:
- domain:Microtubule association is inhibited by the ANK repeats and the Golgi localization region (GoLD).,function:Functions as a cytoplasmic linker protein. Involved in TGN-endosome dynamics.,miscellaneous:The N-terminal half is dispensable for proper Golgi targeting, whereas the GoLD region is required.,similarity:Contains 2 CAP-Gly domains.,similarity:Contains 3 ANK repeats.,subcellular location:Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.,subunit:Homodimer.,
- 细胞定位:
- Cell membrane ; Lipid-anchor . Cytoplasm . Golgi apparatus, Golgi stack . Localized to Golgi stacks as well as on tubulovesicular elements juxtaposed to Golgi cisternae.
![](/uploadfiles/PIC/yt0969-wb.jpg)
- Western Blot analysis of various cells using CLIP3 Polyclonal Antibody
![](/uploadfiles/PIC/yt0969_pic667918522.jpg)
- Immunofluorescence analysis of A549 cells, using CLIP3 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0969_pic668018522.jpg)
- Western blot analysis of lysates from A549 cells, using CLIP3 Antibody. The lane on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/38-IHC-biantaotimm202207.jpg)
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).