CLIP-170 Polyclonal Antibody
- 货号:YT0968
- 应用:WB;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>mTOR signaling pathway
- 蛋白名称:
- CAP-Gly domain-containing linker protein 1
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CLIP1. AA range:1291-1340
- 特异性:
- CLIP-170 Polyclonal Antibody detects endogenous levels of CLIP-170 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:20000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CLIP1;CYLN1;RSN;CAP-Gly domain-containing linker protein 1;Cytoplasmic linker protein 1;Cytoplasmic linker protein 170 alpha-2;CLIP-170;Reed-Sternberg intermediate filament-associated protein;Restin
- 背景:
- The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
- 功能:
- function:Seems to be a intermediate filament associated protein that links endocytic vesicles to microtubules.,similarity:Contains 2 CAP-Gly domains.,subcellular location:Associated with the cytoskeleton.,tissue specificity:Highly expressed in the Reed-Sternberg cells of Hodgkin's disease.,
- 细胞定位:
- Cytoplasm . Cytoplasm, cytoskeleton . Cytoplasmic vesicle membrane ; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle . Localizes to microtubule plus ends (PubMed:21646404, PubMed:17889670). Localizes preferentially to the ends of tyrosinated microtubules (By similarity). Accumulates in plasma membrane regions with ruffling and protrusions. Associates with the membranes of intermediate macropinocytic vesicles (PubMed:12433698). .
- 组织表达:
- Detected in dendritic cells (at protein level). Highly expressed in the Reed-Sternberg cells of Hodgkin disease.
![](/uploadfiles/PIC/yt0968-wb.jpg)
- Western Blot analysis of various cells using CLIP-170 Polyclonal Antibody
![](/uploadfiles/PIC/yt0968-wb(2).jpg)
- Western Blot analysis of RAW264.7 cells using CLIP-170 Polyclonal Antibody
![](/uploadfiles/PIC/yt0968_pic667818522.jpg)
- Western blot analysis of lysates from HUVEC, COLO, MCF-7, HepG2, and RAW264.7 cells, using CLIP1 Antibody. The lane on the right is blocked with the synthesized peptide.