CLC-4 Polyclonal Antibody
- 货号:YT0959
- 应用:WB;IF;ELISA
- 种属:Human;Mouse;Rat
- 简介:
- >>Neutrophil extracellular trap formation
- 蛋白名称:
- H(+)/Cl(-) exchange transporter 4
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CLCN4. AA range:221-270
- 特异性:
- CLC-4 Polyclonal Antibody detects endogenous levels of CLC-4 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CLCN4;H(+)/Cl(-) exchange transporter 4;Chloride channel protein 4;ClC-4;Chloride transporter ClC-4
- 背景:
- chloride voltage-gated channel 4(CLCN4) Homo sapiens The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012],
- 功能:
- function:Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons.,miscellaneous:The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters.,similarity:Belongs to the chloride channel (TC 2.A.49) family.,similarity:Contains 2 CBS domains.,tissue specificity:Abundant in skeletal muscle and also detectable in brain and heart.,
- 细胞定位:
- Early endosome membrane ; Multi-pass membrane protein . Late endosome membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Lysosome membrane ; Multi-pass membrane protein . Recycling endosome membrane ; Multi-pass membrane protein . Localizes to late endosome membrane, lysosome membrane and recycling endosome membrane in the presence of CLCN3. .
- 组织表达:
- Abundant in skeletal muscle and also detectable in brain and heart.
![](/uploadfiles/PIC/yt0959-wb.jpg)
- Western Blot analysis of various cells using CLC-4 Polyclonal Antibody diluted at 1:500
![](/uploadfiles/PIC/yt0959-wb(2).jpg)
- Western Blot analysis of RAT-MUSLE cells using CLC-4 Polyclonal Antibody diluted at 1:500
![](/uploadfiles/PIC/yt0959_pic674918522.jpg)
- Immunofluorescence analysis of HUVEC cells, using CLCN4 Antibody. The picture on the right is blocked with the synthesized peptide.
![](/uploadfiles/PIC/yt0959_pic675018522.jpg)
- Western blot analysis of lysates from MCF-7 cells, using CLCN4 Antibody. The lane on the right is blocked with the synthesized peptide.