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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CHP2 Polyclonal Antibody
- 货号:YT0915
- 应用:WB;ELISA
- 种属:Human;Mouse;Rat
- 蛋白名称:
- Calcineurin B homologous protein 2
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human CHP2. AA range:101-150
- 特异性:
- CHP2 Polyclonal Antibody detects endogenous levels of CHP2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. ELISA: 1:40000. Not yet tested in other applications.
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CHP2;HCA520;Calcineurin B homologous protein 2;Hepatocellular carcinoma-associated antigen 520
- 背景:
- This gene product is a small calcium-binding protein that regulates cell pH by controlling plasma membrane-type Na+/H+ exchange activity. This protein shares sequence similarity with calcineurin B and can bind to and stimulate the protein phosphatase activity of calcineurin A (CnA) and functions in the calcineurin/NFAT (nuclear factor of activated T cells) signaling pathway. Another member of the CHP subfamily, Calcineurin B homologous protein 1, is located on Chromosome 15 and is an inhibitor of calcineurin activity and has a genetic phenotype associated with Parkinson's Disease (OMIM:606988). This gene was initially identified as a tumor-associated antigen and was previously referred to as Hepatocellular carcinoma-associated antigen 520. [provided by RefSeq, Jul 2013],
- 功能:
- function:Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death.,similarity:Contains 4 EF-hand domains.,subunit:Binds to SLC9A1/NHE1.,tissue specificity:Expressed in malignantly transformed cells but not detected in normal tissues.,
- 细胞定位:
- Nucleus . Cytoplasm . Cell membrane . Predominantly localized in a juxtanuclear region. Colocalizes with SLC9A3 in the juxtanuclear region and at the plasma membrane (By similarity). Exported from the nucleus to the cytoplasm through a nuclear export signal (NES) pathway. May shuttle between nucleus and cytoplasm. .
- 组织表达:
- Expressed in malignantly transformed cells but not detected in normal tissues.
![](/uploadfiles/PIC/yt0915-wb.jpg)
- Western Blot analysis of various cells using CHP2 Polyclonal Antibody
![](/uploadfiles/PIC/yt0915_pic666418522.jpg)
- Western blot analysis of lysates from HT-29, K562, and Jurkat cells, using CHP2 Antibody. The lane on the right is blocked with the synthesized peptide.