cGKII Polyclonal Antibody
- 货号：YT0891
- 应用：WB;IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 简介：
- >>cGMP-PKG signaling pathway;>>Gap junction;>>Platelet activation;>>Circadian entrainment;>>Thermogenesis;>>Long-term depression;>>Olfactory transduction;>>Regulation of lipolysis in adipocytes;>>Renin secretion;>>Salivary secretion
- 蛋白名称：
- cGMP-dependent protein kinase 2
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human PKG2. AA range:92-141
- 特异性：
- cGKII Polyclonal Antibody detects endogenous levels of cGKII protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PRKG2;PRKGR2;cGMP-dependent protein kinase 2;cGK 2;cGK2;cGMP-dependent protein kinase II;cGKII
- 背景：
- This gene encodes a protein that belongs to the serine/threonine protein kinase family of proteins. The encoded protein plays a role in the regulation of fluid balance in the intestine. A similar protein in mouse is thought to regulate differentiation and proliferation of cells in the colon. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Binding of cGMP results in enzyme activation.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cGMP subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 cyclic nucleotide-binding domains.,tissue specificity:Highly concentrated in brain, lung and intestinal mucosa.,
- 细胞定位：
- Apical cell membrane ; Lipid-anchor .
- 组织表达：
- Highly concentrated in brain, lung and intestinal mucosa.
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- Western Blot analysis of A549 cells using cGKII Polyclonal Antibody diluted at 1:1000
- Western Blot analysis of 293T cells using cGKII Polyclonal Antibody diluted at 1:1000
- Western blot analysis of lysates from 293 cells, treated with anisomycin 25ug/ml 30', using PKG2 Antibody. The lane on the right is blocked with the synthesized peptide.
- Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
