CdGAP Polyclonal Antibody
- 货号：YT0824
- 应用：IHC;IF;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Rho GTPase-activating protein 31
- 免疫原：
- Synthesized peptide derived from CdGAP . at AA range: 730-810
- 特异性：
- CdGAP Polyclonal Antibody detects endogenous levels of CdGAP protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ARHGAP31;CDGAP;KIAA1204;Rho GTPase-activating protein 31;Cdc42 GTPase-activating protein
- 背景：
- This gene encodes a GTPase-activating protein (GAP). A variety of cellular processes are regulated by Rho GTPases which cycle between an inactive form bound to GDP and an active form bound to GTP. This cycling between inactive and active forms is regulated by guanine nucleotide exchange factors and GAPs. The encoded protein is a GAP shown to regulate two GTPases involved in protein trafficking and cell growth. [provided by RefSeq, Jul 2008],
- 功能：
- developmental stage:Mainly expressed in fetal heart and muscle.,function:Functions as a GTPase-activating protein (GAP) for RAC1 and CDC42. Required for cell spreading, polarized lamellipodia formation and cell migration.,PTM:Phosphorylation on Thr-789 reduces GAP activity.,similarity:Contains 1 Rho-GAP domain.,subunit:Interacts with ITSN1, which inhibits GAP activity. Interacts with PARVA.,
- 细胞定位：
- cytosol,focal adhesion,lamellipodium,
- Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
